Journal: JCI Insight

Article Title: Metalloproteinase PAPP-A regulation of IGF-1 contributes to polycystic kidney disease pathogenesis

doi: 10.1172/jci.insight.135700

Figure Lengend Snippet: (A) Relative mRNA expression of IGF-1 pathway components in kidneys of 7.5-month-old C57BL/6J (n = 4–6) and Pkd1RC/RC mice (n = 5–7). PCR data are expressed relative to Gapdh. (B) Correlation between kidney size (total kidney weight relative to heart weight) and renal Pappa mRNA expression in Pkd1RC/RC mice (n = 15). (C) Pappa mRNA levels in various tissues of WT (n = 3–5) and Pkd1RC/RC mice (n = 4–6). (D) Pappa mRNA levels in WT (n = 6) and Pkd2WS25/– (n = 5) mouse kidneys (16 weeks old). (E) ELISA analysis of PAPP-A protein levels in human ADPKD cystic fluid (n = 6) compared with normal serum reference. (F) Immunolocalization of PAPP-A in normal and ADPKD human kidneys. (G) Western blot analysis of PAPP-A protein levels in normal human RCTE and ADPKD cystic epithelial cells (9-12); graph shows quantification relative to tubulin. Scale bars: 200 μm. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed (for Igf1, 1-tailed) Student’s t test.

Article Snippet: Human ADPKD kidney tissue slides were produced in-house under an institutionally approved IRB protocol, and normal human kidney tissue slides were purchased from Novus Biologicals.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: JCI Insight

Article Title: Metalloproteinase PAPP-A regulation of IGF-1 contributes to polycystic kidney disease pathogenesis

doi: 10.1172/jci.insight.135700

Figure Lengend Snippet: (A) Relative mRNA expression of IGF-1 pathway components in kidneys of 7.5-month-old C57BL/6J (n = 4–6) and Pkd1RC/RC mice (n = 5–7). PCR data are expressed relative to Gapdh. (B) Correlation between kidney size (total kidney weight relative to heart weight) and renal Pappa mRNA expression in Pkd1RC/RC mice (n = 15). (C) Pappa mRNA levels in various tissues of WT (n = 3–5) and Pkd1RC/RC mice (n = 4–6). (D) Pappa mRNA levels in WT (n = 6) and Pkd2WS25/– (n = 5) mouse kidneys (16 weeks old). (E) ELISA analysis of PAPP-A protein levels in human ADPKD cystic fluid (n = 6) compared with normal serum reference. (F) Immunolocalization of PAPP-A in normal and ADPKD human kidneys. (G) Western blot analysis of PAPP-A protein levels in normal human RCTE and ADPKD cystic epithelial cells (9-12); graph shows quantification relative to tubulin. Scale bars: 200 μm. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed (for Igf1, 1-tailed) Student’s t test.

Article Snippet: Human ADPKD kidney tissue slides were produced in-house under an institutionally approved IRB protocol, and normal human kidney tissue slides were purchased from Novus Biologicals.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Cancer Research

Article Title: Blocking Platelet-Derived Growth Factor-D/Platelet-Derived Growth Factor Receptor β Signaling Inhibits Human Renal Cell Carcinoma Progression in an Orthotopic Mouse Model

doi: 10.1158/0008-5472.can-04-4313

Figure Lengend Snippet: Figure 1. PDGF-D is overexpressed in human renal cell carcinoma samples. Normal kidney tissue in the tissue microarray stains with anti–PDGF-D antibody in collecting tubes (A-D). In clear cell type (E and G), granular cell type (F), and granular and clear cell type (H) tumor tissues, PDGF-D stains strongly and homogenously. The arrow indicates positive staining. I, isotype control staining of the tumor tissues. Antigoat immunoglobulin stains negatively of the tumor tissues. Bar, 50 Am.

Article Snippet: Tissue microarray slides bearing 50 human renal cell carcinoma tumor sections and nine normal human kidney sections were purchased from Imgenex (San Diego, CA).

Techniques: Microarray, Staining, Control